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This article in JPR

  1. Vol. 4 No. 2, p. 163-166
     
    Received: Apr 27, 2009


    * Corresponding author(s): yulin.jia@ars.usda.gov
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doi:10.3198/jpr2009.04.0223crmp

Development of Monogenic and Digenic Rice Lines for Blast Resistance Genes Pi-ta, Pi-kh /Pi-ks

  1. Yulin Jia *a and
  2. Karen Moldenhauerb
  1. a USDA–ARS, Dale Bumpers National Rice Research Center, 2890 Hwy. 130 East, Stuttgart, AR 72160
    b Univ. of Arkansas, Rice Research and Extension Center, 2900 Hwy. 130 East, Stuttgart, AR 72160

Abstract

The major blast resistance (R) genes Pi-ta, Pi-ks , Pi-kh have been effectively deployed in rice (Oryza sativa L.) in the southern USA for preventing disease caused by the predominant U.S. races of Magnaporthe oryzae Cav. [=Magnaporthe grisea (Herbert) Barr.]. In the present study, the codominant single nucleotide length polymorphism DNA marker for the Pi-ta gene, and the simple sequence repeat markers, RM144 and RM224, that cosegregate with two alleles of the Pi-k gene, Pi-ks and Pi-kh , were used for R identification in a F10 recombinant inbred line population. This population (Reg. no. MP-3, NSL 452303) was derived from the cross RU9101001/‘Katy’. This population composed of 235 individual lines was jointly released on 24 Apr. 2009 by the USDA–ARS and the University of Arkansas Division of Agriculture Arkansas Agriculture Experiment Station. R gene containing RIL lines were verified with standard pathogenicity assays by means of a set of differential races of M. oryzae in the USA. One hundred eighty-two pure lines were identified from the population with the Pi-ta, Pi-ks , and Pi-kh genes. A total of 56 had Pi-ta and Pi-ks , 51 lines had Pi-ks , 27 had Pi-kh, and 48 lines had Pi-kh and Pi-ta These monogenic and digenic rice lines with the major blast R genes are expected to be useful for studying effects of each R gene singly and in combination for their epistatic interaction with yield and for introducing blast resistance with marker assisted selection.

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