A set of five upland (Gossypium hirsutum L.) cotton germplasm lines, GVS1 (Reg. No. GP-947, PI 663886), GVS2 (Reg. No. GP-948, PI 663887), GVS3 (Reg. No. GP-949, PI 663888), GVS4 (Reg. No. GP-950, PI 663889), and GVS5 (Reg. No. GP-951, PI 663890) were developed and released by the USDA–ARS, Stoneville, MS in August 2009. These five lines include three semi-glanded lines (GVS1, GVS2, GVS3) with varying gland densities and seed gossypol content, one glanded line (GVS4), and one glandless line (GVS5); all five are in the same genetic background Stoneville 7A (PI 529228). In the F11, the seed gossypol content of GVS1, GVS2, and GVS3 was 0.81, 0.49, and 0.50% of the total seed weight, respectively. The parental line Stoneville 7A had 1.54% and Stoneville 7A glandless had 0.00% seed gossypol. The near-isogenic lines GVS4 and GVS5 had 1.51% and 0.00% seed gossypol. GVS1, GVS2, and GVS3 have stem glanding similar to the glanded parent. Calyx glanding is normal in GVS1 and GVS3, whereas GVS2 had a 17% reduction. Boll glanding scores were 67% (GVS1), 60% (GVS2), and 59% (GVS3) of the glanded parent. In the semi-glanded lines, leaf glanding was reduced at the end of the season, but the leaves were always glanded on veins and margins. Yield and fiber properties were measured. These lines will be valuable tools for mapping, for host-plant resistance studies, and as a resource to develop low-seed-gossypol lines.