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This article in SSSAJ

  1. Vol. 20 No. 4, p. 500-504
    Received: Nov 10, 1955

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Tests of Some Methods for Detecting Antibiotics in Soil1

  1. M. Witkamp and
  2. R. L. Starkey2



Various methods of testing soils for antibiotics were compared using soils to which antibacterial substances (penicillin, streptomycin, chloramphenicol) or antifungal substances (cycloheximide, candicidin, nystatin) had been added. Chloramphenicol, penicillin, and cycloheximide could be tested by direct diffusion from soil through a cellulose film into assay agar. All of the antibiotics could be determined in soil-agar mixtures. Whith this method, some antibiotics were more active in nearly neutral media than in acid media. The antifungal antibiotics and streptomycin showed decreasing activity against certain test organisms with an increase in the amount of colloidal material in the soil. There was little or no such effect with chloramphenicol and penicillin.

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