About Us | Help Videos | Contact Us | Subscriptions

Soil Science Society of America Journal Abstract -

Sequence of Products Formed During Denitrification in Some Diverse Western Soils1


This article in SSSAJ

  1. Vol. 27 No. 6, p. 659-662
    Received: Feb 7, 1963
    Accepted: Apr 10, 1963

Request Permissions

  1. Grant S. Cooper and
  2. R. L. Smith2



The sequential products of anaerobic denitrification were determined on seven Western soils (four alkaline, two acid, and one neutral) by soil and gas analysis. The soils with 1% alfalfa and added KNO3 were incubated at moistures slightly greater than field capacity and with an atmosphere of He. The soils and gases were periodically analyzed and balance sheets prepared. The sequence of NO3- → NO2- → N2O → N2 operated in all soils. The N2O and N2 were determined on a gas chromatograph. The rates of nitrogen interchanges and maximal amounts of nitrite, N2O, and N2 were determined. From this data it was postulated that the rate-limiting process for denitrification in acid soils is the reduction of nitrate, while in alkaline soils it is the reduction of nitrite. The reduction of N2O was rapid in all instances. The total time for complete reduction of 300 ppm. N as nitrate to N2 at 30°C. varied only from 28 to 96 hours for the 7 soils. Lowering the temperature to 25° or 20°C. influenced all rates for 3 alkaline soils and resulted in a twofold increase in denitrification time for the 10°C. drop. Decreases in initial concentration of added KNO3 to 150, 75, and 37.5 ppm. did not change the overall rate of dentrification. There was, however, a marked reduction in the maximal amounts of N2O found in the gaseous atmosphere when the initial KNO3 concentration was reduced.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © . Soil Science Society of America