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This article in SSSAJ

  1. Vol. 29 No. 4, p. 393-394
    Received: Nov 20, 1964
    Accepted: Feb 18, 1965

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Gradient Elution of Soil Phosphorus1

  1. Y. Avnimelech and
  2. J. Hagin2



Soil phosphorus was fractionated by the means of gradient elution technique used in chromatography. This method involves a nonequilibrium extraction of the soil P, whereas conventional methods are based on equilibrium extraction. Non-equilibrium extraction is needed in order to describe the fate of the applied P before it reaches equilibrium with the entire soil system.

Soil samples were leached with a solution of linearily increasing acidity. Thus soil P was fractionated according to its solubility in acid. Upon leaching a soil sample, fertilized with superphosphate, two steps in P fixation could be demonstrated. The first step was a rapid one, decreasing the water solubility of the P, yet keeping it soluble in mildly acidic solutions. In the second slower step, P solubility in acidic solutions was decreased.

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