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This article in SSSAJ

  1. Vol. 31 No. 3, p. 436-437
    Received: Nov 14, 1966
    Accepted: Jan 12, 1967

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A Simple Technique for Preparing and Maintaining Sterile Soils for Plant Studies1

  1. R. J. Bartlett and
  2. L. W. Zelazny2



The proposed method depends upon the antimycotic activity of propylene oxide and the permeability of polyethylene. Inside a warmed pressure cooker, the epoxide diffuses rapidly through a plastic bag, sterilizing the moist soil contained. Release of pressure reverses diffusion. Detoxication is completed under vacuum at 45C. The bag then serves as a chamber for the storage of sterile soil or for the sterile culture of plants.

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