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This article in SSSAJ

  1. Vol. 34 No. 2, p. 225-229
    Received: Aug 19, 1969
    Accepted: Oct 2, 1969

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Arylsulfatase Activity of Soils1

  1. M. A. Tabatabai and
  2. J. M. Bremner2



The detection of arylsulfatases (enzymes that hydrolyze aryl sulfates) in soils is reported, and a simple method of assaying soil arylsulfatase activity is described. This method involves colorimetric estimation of the p-nitrophenol released by arylsulfatase activity when soil is incubated with buffered (pH 5.8) potassium p-nitrophenyl sulfate solution and toluene at 37C for 1 hour, and it is rapid, sensitive, and precise. The procedure used to extract p-nitrophenol stops arylsulfatase activity, develops the yellow color used to estimate p-nitrophenol, and gives quantitative recovery of this compound. Studies of the properties of the arylsulfatases in soils are reported.

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