Biosynthesis of Sugars in Soils Incubated with 14C Glucose and 14C Dextran1
- J. M. Oades and
- G. H. Wagner2
Samples of soil were incubated for 28 days after the addition of 14C-labeled glucose or 14C-labeled dextran. Decomposition was monitored by measuring the release of CO2 and determining its radioactivity throughout the incubation period. Soil samples were removed from the incubation at intervals, hydrolyzed to release individual monosaccharides, and the radioactivity of each sugar was measured.
The 14C from glucose or dextran was rapidly incorporated into galactose and mannose during the incubations; but xylose, arabinose, rhamnose and fucose embodied smaller amounts of 14C or none at all.
At the end of 28 days, 75% and 66% of the 14C had been liberated as CO2 from the glucose and dextran incubations, respectively. Of the residual 14C in the soils after 28 days, about 6% from the glucose incubation and 15% from the dextran incubation, was present in saccharides and predominantly found in the hexoses.
A portion of the dextran was apparently protected from decomposition during the incubation.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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