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Abstract

 

This article in SSSAJ

  1. Vol. 37 No. 1, p. 51-52
     
    Received: Feb 28, 1972


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doi:10.2136/sssaj1973.03615995003700010019x

Interactions with Soil Lipase Activation and Inhibition1

  1. Sunil K. Pancholy and
  2. J. Q. Lynd2

Abstract

Abstract

Soil lipase interactions of Psammetic paleustalf, Eufaula loamy sand, were determined with a fluorometric assay of enzymatic cleavage converting nonfluorescent 4-methyl umbelliferone butyrate (4-MUB) to the highly fluorescent 4-methyl umbelliferone (4-MU). Soil lipase activity accelerated with manipulation of base cation levels. Combination effectiveness as reaction activators at 2.0M × 10-4 was Ca + Mg + Na or K or Na + K > Ca + Mg > Ca > Mg > Na or K. Highly significant competitive lipase inhibition was induced with 0.1M Cu2+ > S2- > EDTA at MUB substrate levels of 12.5 to 75.0 × 10-4M. Iron3+ noncompetitive inhibition was near constant.

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