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This article in SSSAJ

  1. Vol. 41 No. 4, p. 733-736
    Received: Dec 27, 1976
    Accepted: Mar 28, 1977

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Microbial Epimerization of Myo-inositol to Chiro-inositol in Soil1

  1. Michael F. L'Annunziata,
  2. Juan Gonzalez I. and
  3. Luis A. Olivares O.2



14Carbon-(1,2,3,5/4,6)-inositol (14C-myo-inositol) was incorporated into an Andosol sandy loam soil and incubated for a 12-day period at 70% field moisture capacity under sterile and nonsterile soil conditions. Comparison by thin-layer chromatography of an isolated metabolite with an authentic standard and infrared spectrometry were used to identify the metabolite as 14C-(1,2,4/3,5,6)-inositol (14C-chiroinositol) of unknown optical activity. The 14C-chiro-inositol was identified in the biologically active soil. No epimerization of 14C-chiroinositol was observed in sterile soil. The abundance of the 14C-chiroinositol was approximately 4% relative to that of the parent compound, as determined by liquid scintillation counting of the two chromatographically separated compounds.

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