Activity of Immobilized Pronase in the Presence of Montmorillonite1
- S. M. Griffith and
- R. L. Thomas2
The activity of soluble and immobilized pronase in the presence of montmorillonite was examined using a differential spectrophotometric technique over a 100-sec incubation period. On the basis of equivalent amounts of enzyme protein, the immobile enzyme had only 28% of the activity of the soluble pronase in the absence of clay. However, in the presence of clay, the immobile pronase was much less inhibited, retaining an average of 75% of its activity while the soluble pronase retained only 31% of its activity. The differential spectrophotometric procedure was satisfactory for monitoring the enzyme action in the presence of suspended clay.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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