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Soil Science Society of America Journal Abstract -

Activity of Immobilized Pronase in the Presence of Montmorillonite1


This article in SSSAJ

  1. Vol. 43 No. 6, p. 1138-1140
    Received: Jan 9, 1978
    Accepted: July 6, 1979

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  1. S. M. Griffith and
  2. R. L. Thomas2



The activity of soluble and immobilized pronase in the presence of montmorillonite was examined using a differential spectrophotometric technique over a 100-sec incubation period. On the basis of equivalent amounts of enzyme protein, the immobile enzyme had only 28% of the activity of the soluble pronase in the absence of clay. However, in the presence of clay, the immobile pronase was much less inhibited, retaining an average of 75% of its activity while the soluble pronase retained only 31% of its activity. The differential spectrophotometric procedure was satisfactory for monitoring the enzyme action in the presence of suspended clay.

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