Laboratory Apparatus for Preparing and Dispensing Nitrogen-15-labeled Anhydrous Ammonia1
- R. J. Norman and
- L. T. Kurtz2
An apparatus and a procedure are described for preparing liquid anhydrous NH3 (LAA) of a desired 15N concentration and a cold-jacketed syringe for dispensing the LAA. Preparation of LAA was accomplished by reacting 15N-labeled (NH4)2SO4 (30-50 atom % 15N) with a 2 M NaOH solution and allowing the resultant 15N-labeled NH3 gas to mix with reagent grade anhydrous NH3 gas dispensed from a lecture bottle (38.5- by 5.1-cm cylinder). The resulting gas mixture was condensed and drained into a collection vessel from which it could be withdrawn, measured, and dispensed with specially built cold-jacketed syringes. With the apparatus and procedure described here, 10 mL of LAA containing 0.7 to 2.0 atom % 15N can be prepared and applied quantitatively in amounts ranging from 25 (15.21 mg NH3-N) to 500 µL (296.98 mg NH3-N). A detailed description of how to construct and operate the 15N-labeled LAA preparation apparatus and dispensing syringes are discussed.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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