Field Denitrification Estimation by Nitrogen-15 and Acetylene Inhibition Techniques1
- A. R. Mosier,
- W. D. Guenzi and
- E. E. Schweizer2
Direct quantification of denitrification N loss from N-fertilized soils has been a long standing problem in soil N research. Recently 15N or acetylene inhibition methods have been used to make in-field estimates of N loss from the soil resulting from denitrification. both methods have potential problems that may affect the validity of the data acquired. A field study was conducted to compare the acetylene inhibition approach with a 15N technique for measuring total N evolution (N2O + N2) from a N-fertilized soil. Miniplots were established in an unplanted clay loam soil and fertilized with 15N-labeled ammonium sulfate. Total N emissions were followed during three irrigation and subsequent drying events. The total N emissions measured by the acetylene method averaged 12 kg N ha−1 compared to 9 kg N ha−1 measured by the 15N technique from the nonacetylene-treated plots. This difference between methods was small relative to the spatial variability observed in measuring gaseous N emissions in the field.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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