Storage Effects on Identification of Rhizobia in Soybean Nodules by Elisa1
- T. A. Matheny,
- M. J. Kasperbauer and
- P. G. Hunt2
Serological analyses of rhizobia in soybean [Glycine max (L.) Merr.] nodules are time-consuming and often must be delayed for extended periods. The objective of this study was to assess the impact of drying and long-term storage on identification of rhizobia in soybean nodules by indirect enzyme-linked immunosorbent assay (ELISA). Nodules formed on ‘Coker 338’ by Bradyrhizobium japonicum strains USDA 3I1b110, NC1004, and B587 were either air-dried at 40°C or freeze-dried, and then stored for 1 month at either ambient temperature, 5, −5 or −60°C. Rhizobia within nodules from all drying and storage combinations were suitable for identification by ELISA. Results of this study indicate that large numbers of nodules can be collected and analyzed for serological identification of rhizobia at a later date, without loss of accuracy.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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