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Abstract

 

This article in SSSAJ

  1. Vol. 51 No. 1, p. 264-265
     
    Received: July 3, 1986


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doi:10.2136/sssaj1987.03615995005100010056x

Storage Effects on Identification of Rhizobia in Soybean Nodules by Elisa1

  1. T. A. Matheny,
  2. M. J. Kasperbauer and
  3. P. G. Hunt2

Abstract

Abstract

Serological analyses of rhizobia in soybean [Glycine max (L.) Merr.] nodules are time-consuming and often must be delayed for extended periods. The objective of this study was to assess the impact of drying and long-term storage on identification of rhizobia in soybean nodules by indirect enzyme-linked immunosorbent assay (ELISA). Nodules formed on ‘Coker 338’ by Bradyrhizobium japonicum strains USDA 3I1b110, NC1004, and B587 were either air-dried at 40°C or freeze-dried, and then stored for 1 month at either ambient temperature, 5, −5 or −60°C. Rhizobia within nodules from all drying and storage combinations were suitable for identification by ELISA. Results of this study indicate that large numbers of nodules can be collected and analyzed for serological identification of rhizobia at a later date, without loss of accuracy.

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