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This article in SSSAJ

  1. Vol. 51 No. 4, p. 941-946
     
    Received: Aug 13, 1986


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doi:10.2136/sssaj1987.03615995005100040022x

Diversity within Iowa Bradyrhizobium japonicum Serogroup 1231

  1. W. J. Hickey,
  2. T. E. Loynachan,
  3. A. Ayanaba and
  4. R. M. Zablotowicz2

Abstract

Abstract

Bradyrhizobium japonicum serogroup 123 dominate soybean [Glycine max (L.) Merr.] nodules in the midwestern USA, but the diversity within this group has been little characterized. This study examined three collections of serogroup 123: nine USDA serogroup 123 strains (representing organisms from several states), 98 isolates collected from several counties and soils across Iowa, and 78 isolates from an intensively sampled 22.8-m2 area in central Iowa. The organisms were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins (SDS-PAGE) and growth response to antibiotics, arginine, and succinate. Of the two methods, SDS-PAGE was more useful for differentiating organisms. By using SDS-PAGE, 74 strains were identified from 176 Iowa serogroup 123 isolates. No relation was evident between strain distribution and soil characteristics or geography. Strain USDA 123 was found not to be representative of the Iowa isolates.

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