Metabolism of Carbamothioate Herbicide, EPTC, by Rhodococcus Strain JE1 Isolated from Soil
- R. O. Ankumah,
- W. A. Dick and
- G. McClung
Biodegradation of the her bicide EPTC (s-ethyl dipropylcarbamothioate) at greatly enhanced rates, which may lead to poor weed control, can occur in soil after successive annual applications. The mechanisms of EPTC degradation by a strain of Rhodococcus sp. (designated JE1) previously isolated from soil were evaluated. Degradation of 14C-labeled (at the 1-propyl position) and unlabeled EPTC was followed by thin layer chromatogaphy and gas chromatography-mass spectrometry. The N-depropyl EPTC and EPTC sulfoxide were identified as initial metabolites formed, apparently via hydroxylation of the α-propyl C and oxidation of the S atom of EPTC, respectively. Other products of EPTC degradation identified were propionaldehyde, s-ethyl carbonothioic acid, s-methyl carbonothioic acid, and traces of propylamine. It is proposed that the soil isolate Rhodococcus JE1 degrades EPTC via either an initial hydroxylation or sulfoxidation reaction, producing metabolites that can be further mineralized to form CO2. The α-propyl hydroxylation reaction, however, is thought to be the dominant pathway whereby degradation of EPTC occurs in soil at enhanced rates.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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