Characterization of the Hydrophobic Acid Fraction Isolated from a Wheat Straw Extract
- Paul R. Grossl and
- William P. Inskeep
The hydrophobic acid portion (WSE-FA) was isolated from a water-soluble extract of wheat (Triticum aestivum L.) straw and chemically characterized. This material was isolated and concentrated on a column containing Amberlite XAD-8 resin. Chemical analysis of the WSE-FA included elemental composition (C, O, H, N, S, P, and ash); titrametric evaluation of carboxylic acid and phenolic functional group content; molecular weight (MW) determination by vapor pressure osmometry (VPO); gel filtration and ultrafiltration; and UV-visible, solid-state cross-polarization magic angle spinning 13C nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy. The MW of the WSE-FA was approximately 600 daltons. The 13C-NMR spectrum, the low MW relative to other humic substances, high E4/E6 ratio, and the low N content suggested that the WSE-FA was comprised of small, aromatic phenolic acids, oxidized polyphenols with exposed carboxyl groups, and saccharides that were condensed during senescence.
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