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Soil Science Society of America Journal Abstract - DIVISION S-3-SOIL BIOLOGY & BIOCHEMISTRY

Mineralization of Amino Acids Applied to Soils Impact of Soil Sieving, Storage, and Inorganic Nitrogen Additions


This article in SSSAJ

  1. Vol. 63 No. 5, p. 1199-1206
    Received: Aug 19, 1998

    * Corresponding author(s): d.jones@bangor.ac.uk
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  1. David L. Jones *a and
  2. David Shannona
  1.  aSchool of Agricultural and Forest Sciences, Univ. Wales-Bangor, Gwynedd, LL57 2UW, UK


The effect of inorganic N additions on the biodegradation and microbial use of organic N pools in soil is poorly understood. To examine the effects of inorganic N on the mineralization rates of amino acids, four soils under contrasting management regimes were subjected to increasing loadings of NH4NO3, ranging from 0 to 120 kg N ha−1 In addition, the effect of soil sieving and storage temperature and time on amino acid mineralization was also investigated. At times ranging from 1 to 40 d after the addition of the inorganic N, the mineralization kinetics of an equimolar mixture of fifteen 14C-labeled amino acids was followed for a subsequent 24-h period. The rate of 14CO2 evolution was soil dependent, with half-lives ranging from 2 h for topsoils to 25 h for subsoils. For all soils, at all times, and at all inorganic-N loadings, the addition of inorganic N appeared to have little effect on the mineralization kinetics of the amino acids to 14CO2 In addition, the presence of inorganic N also had no major effect on the C use efficiency of the microbial biomass. It is speculated that N release from the amino acids into the soil by the microbial biomass may also be little affected by inorganic-N additions. Sieving and storage of soil at either 4 or 18°C for up to 40 d had little impact on amino acid mineralization rate. Experiments with potential microbial disrupting agents (autoclaving, CHCl3 fumigation, HgCl2, and freeze–thaw) all indicated that the observed mineralization of amino acid C was due to microbial activity. We conclude therefore that inorganic N and soil storage has little effect on the microbial use of readily assimilatable amino acids.

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