Molecular Cloning and Linkage Mapping of Cryptochrome Multigene Family in Soybean
- Hisakazu Matsumura,
- Hiroshi Kitajima,
- Shinji Akada,
- Jun Abe,
- Nobuhiro Minaka and
- Ryoji Takahashi
H. Matsumura, Graduate School of Life and Environmental Sciences, Univ. of Tsukuba, Tsukuba, Ibaraki, 305-8518 Japan; H. Kitajima, Faculty of Agriculture and Life Science, Hirosaki Univ., Hirosaki, 036-8561 Japan; S. Akada, Gene Research Center, Hirosaki Univ., Hirosaki, 036-8561 Japan; J. Abe, Research Faculty of Agriculture, Hokkaido Univ., Sapporo, 060-8589 Japan; N. Minaka, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, 305-8604 Japan; R. Takahashi, Graduate School of Life and Environmental Sciences, Univ. of Tsukuba, Tsukuba, Ibaraki, 305-8518 Japan, and National Institute of Crop Science, Tsukuba, Ibaraki, 305-8518 Japan. DDBJ accession number: From AB498928 to AB498938, cDNA clones GmCRY1a, GmCRY1b1, GmCRY1b2, GmCRY1c1, GmCRY1c2, GmCRY1d1, GmCRY1d2, GmCRY2a1, GmCRY2a2, GmCRY2b and GmCRY2c, respectively.
The cryptochromes are a family of blue light photoreceptors that play important roles in the controls of plant development. Seven full-length cryptochrome cDNAs (GmCRY1a, GmCRY1b, GmCRY1c, GmCRY1d, GmCRY2a, GmCRY2b, and GmCRY2c) were isolated by cDNA library screening and reverse transcriptase–polymerase chain reaction from ‘Williams’ soybean [Glycine max (L.) Merr.], indicating that soybean cryptochrome genes comprise a multigene family. They had homologies ranging from 60 to 89% with CRY1 and CRY2 genes of Arabidopsis and pea (Pisum sativum L.). Two types of transcripts were isolated in GmCRY1b, GmCRY1c, GmCRY1d, and GmCRY2a. One type was derived from four exons, whereas the other type was derived from five exons. Occurrence of the former transcript could be explained by retention of the fourth intron, suggesting existence of alternative splicing. Gene sequences were compared between a soybean line, Tokei 780, and an accession of soybean wild relative, Glycine soja, Hidaka 4. Based on a 10-bp indel, an amplicon length polymorphism (ALP) marker was designed for mapping of GmCRY2b. For mapping of the other cryptochrome genes, derived cleaved amplified polymorphic sequence (dCAPS) markers were constructed. The cryptochrome genes were individually assigned to different molecular linkage groups (MLG) (GmCRY1a: MLG C1; GmCRY1b: C2; GmCRY1c: B2; GmCRY1d: F; GmCRT2a: O; GmCRY2b: D1b; GmCRY2c: I). The distribution of cryptochrome genes that was deduced from the soybean genome database was consistent with mapping results.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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