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This article in TPG

  1. Vol. 3 No. 2, p. 69-80
    unlockOPEN ACCESS
    Received: Apr 23, 2010
    Accepted: Sept 15, 2010

    * Corresponding author(s): jose.gonzalez@sdstate.edu
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Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop

  1. Kristene Gedye,
  2. Jose Gonzalez-Hernandez ,
  3. Yuguang Ban,
  4. Xijin Ge,
  5. Jyothi Thimmapuram,
  6. Fengjie Sun,
  7. Chris Wright,
  8. Shahjahan Ali,
  9. Arvid Boe and
  10. Vance Owens
  1. K. Gedye, J. Gonzalez-Hernandez, A. Boe, and V. Owens, Department of Plant Sciences, South Dakota State Univ., Brookings, SD 57007; Y. Ban and X. Ge, Dep. of Mathematics and Statistics, South Dakota State Univ., Brookings, SD 57007; J. Thimmapuram, F. Sun, and C. Wright, W.M. Keck Center for Comparative and Functional Genomics, Roy J. Carver Biotechnology Center, Univ. of Illinois at Urbana-Champaign, Urbana, IL 61801; S. Ali, Biosciences Core Lab.-Genomics, King Abdullah Univ. of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.


Prairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST–SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.

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