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The Plant Genome Abstract - Original Research

Fine Mapping of the SCN Resistance Locus rhg1-b from PI 88788


This article in TPG

  1. Vol. 3 No. 2, p. 81-89
    unlockOPEN ACCESS
    Received: Feb 10, 2010
    Accepted: Sept 30, 2010

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  1. Myungsik Kim,
  2. David L. Hyten,
  3. Andrew F. Bent and
  4. Brian W. Diers 
  1. M. Kim and B.W. Diers, Dep. of Crop Sciences, Univ. of Illinois, Urbana, IL 61801; D.L. Hyten, USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD 20705; A. Bent, Dep. of Plant Pathology, Univ. of Wisconsin, Madison, WI 53706.


Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fine-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F2 and F3 plants and F3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to identify recombination events close to rhg1-b. Lines developed from these recombinant plants were then tested for resistance to the SCN isolate PA3, which originally had an HG type 0 phenotype, and with genetic markers. Analysis of lines carrying key recombination events positioned rhg1-b between the simple sequence repeat (SSR) markers BARCSOYSSR_18_0090 and BARCSOYSSR_18_0094. This places rhg1-b to a 67-kb region of the ‘Williams 82’ genome sequence. The receptor-like kinase gene that has been previously identified as a candidate for the ‘Peking’-derived SCN resistant rhg1 gene is adjacent to, but outside of, the rhg1-b interval defined in the present study.

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