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This article in TPG

  1. Vol. 4 No. 1, p. 12-23
    unlockOPEN ACCESS
     
    Received: Aug 1, 2010
    Accepted: Jan 27, 2011


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doi:10.3835/plantgenome2010.08.0018

Abiotic Stress-Related Expressed Sequence Tags from the Diploid Strawberry Fragaria vesca f. semperflorens

  1. Maximo. Rivarola,
  2. Agnes P. Chan,
  3. David E. Liebke,
  4. Admasu. Melake-Berhan,
  5. Hui. Quan,
  6. Foo. Cheung,
  7. Shu. Ouyang,
  8. Kevin M. Folta,
  9. Janet P. Slovin  and
  10. Pablo D. Rabinowicz*
  1. M. Rivarola, D.E. Liebke, and P.D. Rabinowicz, Institute for Genome Sciences, Univ. of Maryland, School of Medicine, BioPark Building II, 801 West Baltimore St., Baltimore, MD 21201; P.D. Rabinowicz, Dep. of Biochemistry and Molecular Biology, Univ. of Maryland, School of Medicine, BioPark Building II, 801 West Baltimore St., Baltimore, MD 21201 A.P. Chan, A. Melake-Berhan, H. Quan, F. Cheung, S. Ouyang, and P.D. Rabinowicz, The J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD 20850; K.M. Folta, Horticultural Sciences Dep. and the Graduate Program in Plant Molecular and Cellular Biology, 1301 Fifield Hall, Univ. of Florida, Gainesville, FL 32611; J.P. Slovin, USDA-ARS, Genetic Improvement of Fruits and Vegetables Lab. Henry A. Wallace Beltsville Agricultural Research Center, 10300 Baltimore Ave., Beltsville, MD 20705.

Abstract

Strawberry (Fragaria spp.) is a eudicotyledonous plant that belongs to the Rosaceae family, which includes other agronomically important plants such as raspberry (Rubus idaeus L.) and several tree-fruit species. Despite the vital role played by cultivated strawberry in agriculture, few stress-related gene expression characterizations of this crop are available. To increase the diversity of available Fragaria transcriptome sequence, we produced 41,430 Fragaria vesca L. expressed sequence tags (ESTs) from plants growing under water-, temperature-, and osmotic-stress conditions as well as a combination of heat and osmotic stresses that is often found in irrigated fields. Clustering and assembling of the ESTs resulted in a total of 11,836 contigs and singletons that were annotated using Gene Ontology (GO) terms. Furthermore, over 1200 sequences with no match to available Rosaceae ESTs were found, including six that were assigned the “response to stress” GO category. Analysis of EST frequency provided an estimate of steady state transcript levels, with 91 sequences exhibiting at least a 20-fold difference between treatments. This EST collection represents a useful resource to advance our understanding of the abiotic stress-response mechanisms in strawberry. The sequence information may be translated to valuable tree crops in the Rosaceae family, where whole-plant treatments are not as simple or practical.

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