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The Plant Genome Abstract - Original Research

An Active CACTA-Family Transposable Element is Responsible for Flower Variegation in Wild Soybean Glycine soja


This article in TPG

  1. Vol. 5 No. 2, p. 62-70
    unlockOPEN ACCESS
    Received: Nov 6, 2011

    * Corresponding author(s): masako@affrc.go.jp
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  1. Ryoji Takahashi a,
  2. Yasumasa Moritab,
  3. Masayoshi Nakayamab,
  4. Akira Kanazawac and
  5. Jun Abec
  1. a National Institute of Crop Science, Kannondai, Tsukuba, Ibaraki, 305-8518 Japan
    b National Institute of Floricultural Science, Fujimoto, Tsukuba, Ibaraki, 305-8519 Japan
    c Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589 Japan


A plant producing flowers with purple and white variegation was discovered in an accession of Glycine soja Siebold & Zucc. that was introduced from Russia. The mutant line was designated as B00146-m. Lines with white flowers (B00146-w) and purple flowers (B00146-r) were developed from the progeny of B00146-m. The flower color was controlled by the W1 locus encoding a flavonoid 3′5′-hydroxylase (F3′5′H). The allele for variegated flowers was designated as w1-m. The gene symbol was approved by the Soybean Genetics Committee. Polymerase chain reaction (PCR) suggested that a DNA fragment with a molecular size of ∼3.9 kb was inserted in the first exon of the F35H gene in B00146-m whereas such insertion was not observed in B00146-w and B00146-r. These results suggested that an active mobile element was inserted in the first exon and was responsible for flower variegation. The inserted fragment was identified as a 3883 bp long CACTA-family transposable element and it was designated as Tgs1. Similarity of overall sequence and terminal inverted repeats suggested that Tgs1 and the soybean lectin gene transposable element Tgm1 make up a subgroup. Frequency of germinal reversion was low probably due to the integration into an exon. Tgs1 had a truncated version of the transposase gene and may be a nonautonomous element.

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