About Us | Help Videos | Contact Us | Subscriptions
 

Abstract

 

This article in TPG

  1. Vol. 5 No. 3, p. 114-125
    unlockOPEN ACCESS
     
    Received: June 16, 2012
    Published: December 12, 2012


    * Corresponding author(s): Jeff_Maughan@byu.edu
 View
 Download
 Alerts
 Permissions
Request Permissions
 Share

doi:10.3835/plantgenome2012.06.0011

Single Nucleotide Polymorphism Identification, Characterization, and Linkage Mapping in Quinoa

  1. P. J. Maughan a,
  2. S. M. Smitha,
  3. J. A. Rojas-Beltránb,
  4. D. Elzingaa,
  5. J. A. Raneya,
  6. E. N. Jellena,
  7. A. Bonifacioc,
  8. J. A. Udalla and
  9. D. J. Fairbanksd
  1. a Brigham Young Univ., Provo, UT 84602
    b Universidad Mayor de San Simón, Cochabamba, Bolivia
    c Fundacion PROINPA, Casilla Postal 4285, Cochabamba, Bolivia
    d Utah Valley Univ., Orem, UT 84058

Abstract

Quinoa (Chenopodium quinoa Willd.) is an important seed crop throughout the Andean region of South America. It is important as a regional food security crop for millions of impoverished rural inhabitants of the Andean Altiplano (high plains). Efforts to improve the crop have led to an increased focus on genetic research. We report the identification of 14,178 putative single nucleotide polymorphisms (SNPs) using a genomic reduction protocol as well as the development of 511 functional SNP assays. The SNP assays are based on KASPar genotyping chemistry and were detected using the Fluidigm dynamic array platform. A diversity screen of 113 quinoa accessions showed that the minor allele frequency (MAF) of the SNPs ranged from 0.02 to 0.50, with an average MAF of 0.28. Structure analysis of the quinoa diversity panel uncovered the two major subgroups corresponding to the Andean and coastal quinoa ecotypes. Linkage mapping of the SNPs in two recombinant inbred line populations produced an integrated linkage map consisting of 29 linkage groups with 20 large linkage groups, spanning 1404 cM with a marker density of 3.1 cM per SNP marker. The SNPs identified here represent important genomic tools needed in emerging plant breeding programs for advanced genetic analysis of agronomic traits in quinoa.

  Please view the pdf by using the Full Text (PDF) link under 'View' to the left.

Copyright © 2012. Copyright © 2012 Crop Science Society of America