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The Plant Genome Abstract - Special Submissions – Legume Genomics

Genetic Mapping and Quantitative Trait Loci Analysis for Disease Resistance Using F2 and F5 Generation-based Genetic Maps Derived from ‘Tifrunner’ × ‘GT-C20’ in Peanut


This article in TPG

  1. Vol. 6 No. 3
    unlockOPEN ACCESS
    Received: May 28, 2013
    Published: September 13, 2013

    * Corresponding author(s): Baozhu.Guo@ars.usda.gov
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  1. Hui Wangabch,
  2. Manish K. Pandeybcd,
  3. Lixian Qiaobce,
  4. Hongde Qinf,
  5. Albert K. Culbreathb,
  6. Guohao Heg,
  7. Rajeev K. Varshneyd,
  8. Brian T. Scullyc and
  9. Baozhu Guo *c
  1. a Fujian Agricultural and Forestry Univ., College of Plant Protection, Fuzhou, China
    b Univ. of Georgia, Dep. of Plant Pathology, Tifton, GA 31793
    c USDA-ARS, Crop Protection and Management Research Unit, Tifton, GA 31793
    h Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, China. Hui Wang and Manish K. Pandey contributed equally to this work
    d International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India
    e Qingdao Agricultural Univ., College of Life Science, Qingdao, China
    f Hubei Academy of Agricultural Sciences, Wuhan, China
    g Tuskegee Univ., Tuskegee, AL 36088


One mapping population derived from Tifrunner × GT-C20 has shown great potential in developing a high density genetic map and identifying quantitative trait loci (QTL) for important disease resistance, tomato spotted wilt virus (TSWV) and leaf spot (LS). Both F2 and F5 generation-based genetic maps were previously constructed with 318 and 239 marker loci, respectively. Higher map density could be achieved with the F2 map (5.3 cM per locus) as compared to the F5 (5.7 cM per locus). Quantitative trait loci analysis using multi-environment phenotyping data from F8 and higher generations for disease resistance identified 54 QTL in the F2 map including two QTL for thrips (12.14–19.43% phenotypic variation explained [PVE]), 15 for TSWV (4.40–34.92% PVE), and 37 for LS (6.61–27.35% PVE). Twenty-three QTL could be identified in the F5 map including one QTL for thrips (5.86% PVE), nine for TSWV (5.20–14.14% PVE), and 13 for LS (5.95–21.45% PVE). Consistent QTL identified in each map have shown higher phenotypic variance than nonconsistent QTL. As expected, the number of QTL and their estimates of phenotypic variance were lower in the F5 map. This is the first QTL study reporting novel QTL for thrips, TSWV, and LS in peanut (Arachis hypogaea L.), and therefore, future studies will be conducted to refine these QTL.

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