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The Plant Genome Abstract - Original Research

Genome-Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection


This article in TPG

  1. Vol. 9 No. 3
    unlockOPEN ACCESS
    Received: Jan 20, 2016
    Accepted: Mar 23, 2016
    Published: July 28, 2016

    * Corresponding author(s): maricelis.acevedo@ndsu.edu
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  1. Meriem Aouna,
  2. Matthew Breilanda,
  3. M. Kathryn Turnerc,
  4. Alexander Loladzeb,
  5. Shiaoman Chaod,
  6. Steven S. Xue,
  7. Karim Ammarb,
  8. James A. Andersonc,
  9. James A. Kolmerf and
  10. Maricelis Acevedo *a
  1. a Dep. Plant Pathology, North Dakota State Univ., Fargo ND, 58108, USA
    c Dep. Agronomy and Plant Genetics, Univ. of Minnesota, St Paul, MN, 55108, USA
    b International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, D.F., Mexico
    d USDA–ARS Genotyping Lab., Biosciences Research Lab., Fargo, ND, 58108, USA
    e USDA–ARS, Cereal Crops Research Unit, Northern Crop Science Laboratory, Fargo, ND, USA
    f USDA–ARS Cereal Disease Laboratory, St. Paul, MN, 55108, USA
Core Ideas:
  • Thirteen durum wheat accessions showed resistance to all Puccinia triticina races tested
  • GWAS revealed 88 SNPs (37 loci) associated with leaf rust response in durum wheat
  • Associations were identified on all chromosomes except 1B and 7B
  • GWAS revealed 14 previously uncharacterized loci for leaf rust resistance


Leaf rust (caused by Puccinia triticina Erikss. [Pt]) is increasingly impacting durum wheat (Triticum turgidum L. var. durum) production with the recent appearance of races with virulence to widely grown cultivars in many durum producing areas worldwide. A highly virulent P. triticina race on durum wheat was recently detected in Kansas. This race may spread to the northern Great Plains, where most of the US durum wheat is produced. The objective of this study was to identify sources of resistance to several races from the United States and Mexico at seedling stage in the greenhouse and at adult stage in field experiments. Genome-wide association study (GWAS) was used to identify single-nucleotide polymorphism (SNP) markers associated with leaf rust response in a worldwide durum wheat collection of 496 accessions. Thirteen accessions were resistant across all experiments. Association mapping revealed 88 significant SNPs associated with leaf rust response. Of these, 33 SNPs were located on chromosomes 2A and 2B, and 55 SNPs were distributed across all other chromosomes except for 1B and 7B. Twenty markers were associated with leaf rust response at seedling stage, while 68 markers were associated with leaf rust response at adult plant stage. The current study identified a total of 14 previously uncharacterized loci associated with leaf rust response in durum wheat. The discovery of these loci through association mapping (AM) is a significant step in identifying useful sources of resistance that can be used to broaden the relatively narrow leaf rust resistance spectrum in durum wheat germplasm.

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