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The Plant Genome Abstract - Original Research

Identification and Characterization of Quantitative Trait Loci for Shattering in Japonica Rice Landrace Jiucaiqing from Taihu Lake Valley, China

 

This article in TPG

  1. Vol. 9 No. 3
    unlockOPEN ACCESS
     
    Received: Mar 30, 2016
    Accepted: Aug 28, 2016
    Published: September 29, 2016


    * Corresponding author(s): wzf@njau.edu.cn
    hszhang@njau.edu.cn
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doi:10.3835/plantgenome2016.03.0034
  1. Jinping Chenga,
  2. Yongqi Hea,
  3. Chengfang Zhana,
  4. Bin Yanga,
  5. Enshun Xua,
  6. Hongsheng Zhang *a and
  7. Zhoufei Wang *a
  1. a The Laboratory of Seed Science and Technology, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, PR China

Abstract

Easy shattering reduces yield from grain loss during rice (Oryza sativa L.) harvest. We characterized a nonshattering japonica rice landrace Jiucaiqing from Taihu Lake valley in China. The breaking tensile strength (BTS; grams force, gf) of the grain pedicel was measured using a digital force gauge to evaluate the degree of shattering at 0, 7, 14, 21, 28, and 35 d after heading (DAH). The BTS of Jiucaiqing did not significantly decrease with increasing DAH, maintaining a level of 152.2 to 195.9 gf, while that of indica IR26 decreased greatly during 0 to 14 DAH and finally stabilized at ∼100 gf. Then the chromosome segment substitution lines (CSSLs) and near isogenic lines (NILs) of Jiucaiqing in IR26 background were developed for quantitative trait loci (QTL) mapping. Four putative QTL (qSH1JCQ, qSH3JCQ, qSH6JCQ, and qSH11JCQ) for shattering were detected, and the qSH1JCQ was confirmed on chromosome 1. We further mapped qSH1JCQ to a 98.4-kb region, which contains 14 genes. Os01g62920 was considered to be a strong candidate for qSH1JCQ, which colocated with qSH1. Further quantitative real-time polymerase chain reaction (PCR) analyses confirmed that the QTL qSH1JCQ can significantly decrease the expression of shattering related genes (qSH1, OsCPL1, Sh4, SH5, and SHAT1) especially at the middle development stage at 10 and 15 cm panicle length, which causes rice shattering decrease. The elite allele and the NIL with desirable agronomic traits identified in this study could be useful for rice breeding.

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