Descriptive statistics of phenotypic response to tar spot complex infection in the Drought Tolerant Maize for Africa panel and three biparental doubled-haploid populations.
| Population | Mean | Min. | Max. | Median | SD | Skewness | Kurtosis | Heritability |
| DTMA | 2.04 | 1.31 | 3.23 | 2.00 | 0.45 | 0.55 | 0.06 | 0.80 |
| Pop1 | 2.33 | 1.81 | 3.00 | 2.30 | 0.23 | 0.49 | −0.07 | 0.54 |
| Pop2 | 2.14 | 1.18 | 3.95 | 2.04 | 0.54 | 0.98 | 1.02 | 0.88 |
| Pop3 | 2.50 | 1.37 | 4.39 | 2.29 | 0.68 | 0.68 | −0.36 | 0.93 |
Single-nucleotide polymorphism (SNP) characteristic information of the Drought Tolerant Maize for Africa panel and three biparental doubled-haploid populations before and after filter of genotyping-by-sequencing datasets.
| Population | No. of SNPs |
Missing rate |
Heterozygosity rate |
|||
| Before filter | After filter | Before filter | After filter | Before filter | After filter | |
| % | ||||||
| DTMA | 955120 | 261055 | 15.12 | 6.36 | 1.53 | 1.86 |
| Pop1 | 955120 | 44146 | 42.62 | 7.68 | 0.51 | 2.32 |
| Pop2 | 955120 | 47203 | 42.32 | 7.90 | 0.47 | 2.55 |
| Pop3 | 955120 | 45775 | 43.57 | 7.60 | 0.33 | 2.13 |
Significantly associated single-nucleotide polymorphisms (SNPs) revealed by association-mapping analysis in the Drought Tolerant Maize for Africa panel on chromosomes 2, 3, and 7, with the top five significantly associated SNPs on chromosome 8 listed.
| SNP name† | Allele | P-value | PVE‡ | MAF§ | Candidate gene | Annotation |
| % | ||||||
| S2_143450705 | A,T | 1.28 × 10−5 | 5.14 | 0.40 | – | – |
| S3_187460281 | A,T | 2.63 × 10−5 | 4.76 | 0.30 | GRMZM2G072853 | – |
| S3_224167844 | A,C | 1.17 × 10−5 | 5.19 | 0.15 | GRMZM2G339540 | Leucine-rich repeat receptor-like protein kinase family protein |
| S3_28621298 | A,G | 2.57 × 10−5 | 4.77 | 0.44 | GRMZM2G180622 | Sarcoplasmic reticulum histidine-rich calcium-binding protein |
| S7_21279210 | A,G | 2.41 × 10−5 | 4.80 | 0.18 | GRMZM2G063340 | Rps12 ribosomal proteins12 |
| S8_83777799 | A,G | 1.29 × 10−11 | 13.00 | 0.25 | – | – |
| S8_83777830 | C,G | 1.33 × 10−11 | 12.98 | 0.27 | – | – |
| S8_83335753 | A,T | 5.73 × 10−11 | 12.11 | 0.23 | GRMZM2G104025 | 60S ribosomal protein L18–3-like |
| S8_82826985 | A,G | 5.83 × 10−11 | 12.1 | 0.24 | – | – |
| S8_80045991 | G,T | 6.11 × 10−11 | 12.07 | 0.23 | GRMZM2G106526 | ATP10 protein |
Linkage-mapping analysis results of tar spot complex resistance in three biparental doubled-haploid populations.
| Population | Chromosome | Left marker (position)† | Right marker (Position) | LOD‡ | PVE§ | Additive effect |
| % | ||||||
| Pop1 | 4 | C4M190 (197840452) | C4M191 (209059831) | 5.82 | 8.63 | 0.07 |
| 7 | C7M315 (144585946) | C7M316 (149528489) | 9.19 | 14.41 | 0.09 | |
| 8 | C8M347 (70713777) | C8M348 (82228021) | 13.18 | 21.85 | 0.12 | |
| Pop2 | 1 | C1M30 (92194874) | C1M31 (100468505) | 9.07 | 17.82 | 0.19 |
| 2 | C2M95 (36770127) | C2M96 (45491073) | 3.59 | 6.19 | 0.11 | |
| 5 | C5M266 (171692383) | C5M267 (174071529) | 3.06 | 5.12 | 0.10 | |
| 8 | C8M390 (46943754) | C8M391 (83381560) | 17.88 | 43.31 | 0.30 | |
| Pop3 | 1 | C1M38 (184932563) | C1M39 (188828594) | 14.07 | 32.29 | 0.38 |
| 7 | C7M366 (129063233) | C7M367 (131806297) | 3.25 | 5.96 | 0.17 | |
| 8 | C8M399 (23160646) | C8M401 (38729344) | 8.24 | 16.78 | 0.28 | |
| 10 | C10M509 (147329967) | C10M511 (147901114) | 3.24 | 6.01 | 0.17 |