Figure 1.
Figure 1.

Secondary leaves inoculated with conidia of DW5, a race 5 isolate produced by the tan spot fungus Pyrenophora tritici-repentis, or infiltrated with Ptr ToxB. A and B: Reaction of Salamouni (A) (average disease reaction type 1.8) and Katepwa (B) (average disease reaction type 3.7) to conidial inoculations with the P. tritici-repentis race 5 isolate DW5. C and D: Reaction of Salamouni (C) and Katepwa (D) infiltrated with Ptr ToxB. The boundaries of the infiltrated regions are marked in black.

 


Figure 2.
Figure 2.

Molecular mapping of the Tsc2 locus in the recombinant inbred population derived from Salamouni × Katepwa. The genetic linkage map of the Tsc2 region (middle) corresponds to the deletion bin 2BS3 0.84–1.00 on the physical map (left). Markers are indicated to the right of the genetic map. Microsatellite simple sequence repeat (SSR) markers are shown in black; sequence-tagged site (STS) and restriction fragment length polymorphism (RFLP) markers derived from bin-mapped expressed sequence tags (ESTs) are shown in blue and burgundy, respectively; the marker XBE444541, which was derived from a bin-mapped EST and initially mapped as an RFLP then converted to an STS marker, is shown in bright red; and markers developed from ESTs identified based on colinearity with rice and Brachypodium distachyon are shown in green. The composite interval regression map of the Tsc2 region after inoculation with the Pyrenophora tritici-repentis race 5 isolate DW5 is on the right. The critical logarithm of odds (LOD) threshold of 3.30 is indicated by the dotted line, and the LOD scale is indicated at the bottom.

 


Figure 3.
Figure 3.

Comparison of the Tsc2 region in wheat chromosome 2BS (middle) with rice chromosome 4 (left) and Brachypodium distachyon chromosome 5 (right). Markers developed based on bin-mapped expressed sequence tags (ESTs) are indicated in black, and markers developed based on colinearity with rice and Brachypodium distachyon are indicated in bright red. The Mb positions of colinear genes are indicated to the left of rice chromosome 4 and to the right of Brachypodium distachyon chromosome 5. Hatched regions of rice chromosome 4 indicate they are not to scale.

 


Figure 4.
Figure 4.

Polyacrylamide (top) and agarose (bottom) gel electrophoresis of polymerase chain reaction (PCR) products amplified from seven Ptr ToxB-insensitive genotypes (lanes 2–8; cultivars Salamouni through ‘Knudson’) and seven Ptr ToxB-sensitive genotypes (lanes 9–15; cultivars Katepwa through 6B662) with the primer set for marker XBE444541. Lanes are annotated across the top. A 1 kb ladder is shown in lanes 1 and 16.

 


Figure 5.
Figure 5.

Histogram of the average tan spot disease reaction types of the Ptr ToxB-insensitive and-sensitive recombinant inbred lines versus frequency in the Salamouni × Katepwa population.