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Library and sequence specifications.

 
Library Accession numbers Sequence attempts High quality ESTs Avg. read length (bp) Cloned cDNA fraction
Heat H4 EX665399–EX674283 9984 8885 649 0.8–4 kb
Cold H4 DY666641–DY676279 10,368 9639 733 1.5 kb (average)
Drought H4 EX674284–EX681129 9984 6846 695 0.8–4 kb
Salt H4 EX656822–EX665398 9984 8577 665 0.8–4 kb
Salt/Heat H4 EX681130–EX688612 10,176 7483 625 0.8–4 kb
Total 50,496 41,430 675
EST, expressed sequence tag.
cDNA, complementary DNA.



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Twenty unique sequences assembled from the highest numbers of expressed sequence tags (ESTs).

 
Unique sequence name Protein sequence match No. of EST components Sequence length (bp) Best match accession No. % Identity % Similarity % Coverage Taxon
CL1Contig 62 Metallothionein-like protein 2 497 1294 P93134 96.25 97.50 100 Fragaria ×ananassa Duchesne ex Rozier
CL1Contig 88 Class I LMW HSP 432 804 AAR25848 83.57 94.29 90.91 Carica papaya L.
CL1Contig 129 Nonspecific lipid transfer protein 310 2443 AAY83341 100 100 100 F. ×ananassa
CL1Contig 68 Nonspecific lipid transfer protein 261 959 AAY83346 93.97 96.55 99.15 F. ×ananassa
CL1Contig 25 RUBISCO SSU 255 1319 AAA33866 86.90 95.24 93.85 Malus domestica Borkh × Pyrus communis L.
CL1Contig 47 Class I LMW HSP 252 850 AAC39360 97.42 98.71 99.36 F. ×ananassa
CL1Contig 1 Metallothionein-like protein 224 881 CAA04766 100 100 100 Fragaria vesca L.
CL3Contig 1 Dehydrin 215 1387 ABG56268 78.65 80.90 38.86 Malus domestica
CL1Contig 60 Hypothetical Protein 183 1505 BAD46202 75.36 79.71 27.82 Oryza sativa L.
CL1Contig 49 Class III Heme Dep. Peroxidase 176 1341 AAA99868 87.50 95.31 96.39 Gossypium hirsutum L.
CL1Contig 15 Peptidylprolyl isomerase 168 2061 CAC84116 92.98 95.32 98.84 Betula pendula Roth
CL1Contig 56 LMW HSP 165 862 ABK92179 81.41 91.03 100 Nelumbo nucifera Gaertn.
CL1Contig 131 Glycine Rich Protein-like 2 158 4731 ABG76000 93.60 96.80 95.82 Gossypium hirsutum
CL1Contig 14 Fragaria a 1 allergen 156 867 CAJ29538 100 100 100 F. ×ananassa
CL1Contig 132 Light-harvesting chl a/b binding protein 153 1120 AAC34983 95.47 98.11 100 Prunus persica (L.) Batsch
CL1Contig 93 Putative Glycine-rich RNA-binding protein 136 1042 AAL13082 86.23 88.02 93.82 Prunus avium (L.) L.
CL4Contig1 LMW HSP20 136 684 ABD32352 84.92 96.03 79.25 Medicago truncatula Gaertn.
CL1Contig64 Class I LMW HSP 129 1086 AAC39360 97.44 98.72 100 F. ×ananassa
CL1Contig98 Light-harvesting chl a/b binding protein LHCII type I 127 996 AAB87573 94.59 97.68 97.37 Panax ginseng C. A. Mey.
CL6Contig1 Auxin-repressed dormancy associated 12.5 KDa protein 126 1061 Q05349 100 100 100 F. ×ananassa
Defined by the best BLASTX match with E < 10−10 against all plant proteins.
LMW HSP, low molecular weight heat shock protein.
§RUBISCO SSU, ribulose 1,5 bisphosphate carboxylase small subunit.
RNA, ribonucleic acid.



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Fragaria vesca stress-response sequences not found in other Rosaceae expressed sequence tag (EST) sets.

 
GenBank accession No. Description Library Biological process
EX673244 Unknown protein Heat H4 Response to oxidative stress
EX686969 BiP luminal binding protein Salt/Heat H4 Protein folding
Response to bacterium
Response to heat
DY673869 ATP dependent DNA Cold H4 DNA repair
Ligase family protein DNA replication
DNA recombination
DY672681 Mre11 (meiotic recombination protein 11) Cold H4 Double-strand break repair
Serine/threonine phosphatase
DY672970 Zinc-binding dehydrogenase family protein Cold H4 Response to cold
DY670217 Unknown protein Cold H4 Heat acclimation