(a) Shattering levels and (b–f) expression levels of shattering related genes regulated by the Jiucaiqing allele of qSH1JCQ in various development stages. The breaking tensile strength (BTS) of grain pedicels was tested at 0, 7, 14, 21, 28, and 35 d after heading. The unit of BTS, gf, is the abbreviation of grams force. The transcript levels were determined with quantitative real-time polymerase chain reaction using gene specific primers when 5, 10, 15, and 20 cm panicle length. Transcript levels were calculated relative to the qSH1 when 5 cm panicle length. Bars indicate the mean values ± standard deviation. Single and double asterisks (* and **) represent significant differences between IR26 and NIL at p < 0.05 and p < 0.01 level, respectively.