Fig. 1.
Fig. 1.

Binding, cleavage, and repair using the CRISPR-Cas9 system. The Cas9 protein from Streptococcus pyogenes (SpyCas9) and associated guide RNA (gRNA), containing a 20-nt recognition sequence, will cleave a target sequence located upstream of the protospacer adjacent motif (PAM) region. The SpyCas9-associated gRNA is highly specific for the 10- to 12-nucleotide seed sequence immediately upstream of the PAM sequence.