Fig. 1.
Fig. 1.

Scatter plot showing the genome-wide linkage disequilibrium (LD) decay over genetic distances. Pair-wise LD r2 value is plotted against the intermarker map distance, which was based on a reference consensus map (Wang et al., 2014). The blue curve represents the fitted model, and the red dashed line shows LD declines to 0.1 at 11 cM.

 


Fig. 2.
Fig. 2.

STRUCTURE analysis revealed six subpopulations (S1–S6). S1, S2, S4, S5, and S6 represent Iranian landrace group 1, eastern European cultivar group, western European cultivar group, east-Asian accession group, and Iranian landrace group 2, respectively. Origins of wheat accessions in S3 were very diverse. Vertical lines represent membership coefficients of accessions corresponding to the six subpopulations.

 


Fig. 3.
Fig. 3.

Distribution of mean leaf rust infection types of 1537 accessions inoculated with Pt2013, Pt52-2, and Pt54-1.

 


Fig. 4.
Fig. 4.

Box plot showing distribution of wheat leaf rust infection types (ITs) in six subpopulations inoculated with Pt2013. The diamond represents mean IT value of each subpopulation.

 


Fig. 5.
Fig. 5.

Inferred locations of single-nucleotide polymorphism (SNP) marker IWA6541, IWA6110, and IWA6129 on the SNP9K consensus map (right) and homeologous sequence corresponding to each marker on the Brachypodium chromosome 2 physical map (left). IWA6129 is tentatively mapped in between IWA6541 and IWA6110.

 


Fig. 6.
Fig. 6.

Chromosome locations of 14 quantitative trait loci (QTL) for leaf rust resistance identified in this study and previously mapped Lr genes and QTL (left side of bars). The vertical lines on the left show genomic regions harboring known genes or QTL. Chromosome lengths were standardized to the same relative length. Marker positions were based on the 90K SNP consensus linkage map. The tag SNP of each QTL was shown on the linkage map, and suggested QTL name was given in parentheses.